EDVOTEK Simulation of HIV Detection Kit
Product Code : SCL-BLE-12007
Please Note: This is a simulated HIV Detection Kit for use in education. It is not suitable for diagnostic or clinical use*
The HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood, they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary antibody that causes a colour change upon addition of substrate.
In this experiment, your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.
Group Size: for 10 lab groups
Storage: Refrigerator
Time Required: 60 minutes
The kit includes:
Full instructions
10X ELISA wash buffer
ELISA dilution buffer
Antigen (lyophilized)
Primary antibody (lyophilized)
Secondary antibody (lyophilized)
ABTS (lyophilized)
ABTS reaction buffer
Microtitre plates
Transfer pipettes
Snap-top microcentrifuge tubes
15ml conical tubes
Learn the biology and methodology of the Enzyme-Linked Immunosorbent Assay (ELISA)
Perform an indirect (two-step) ELISA with controls and patient samples
Experiment replicates a clinical screen to detect HIV antibodies in patient blood samples
Understand the molecular biology of HIV and the pathogenesis of AIDS
GCSE Biology: HIV Virus Detection
A-Level Biology: ELISA & Immunoassays, HIV Virus Detection
Full supporting materials included.
EDVOTEK YouTube Channel is full of useful resources.
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