EDVOTEK Transformation of E-Coli (GFP) Kit
Product Code : SCL-BLE-12009
In this Transformation of E-Coli Kit, students will explore the biological process of bacterial transformation using E-Coli and green fluorescent protein plasmid DNA.
At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.
Group Size: for 10 lab groups
Storage: some components require refrigerator and freezer storage upon receipt
Time Required: Set-up - 50 minutes, Incubation - overnight, Transformation - 15 minutes
The kit includes:
Full instructions
BactoBeads™ E.coli GFP Host
Supercoiled pFluoroGreen™ plasmid DNA
Ampicillin
IPTG
Calcium Chloride
Growth Additive
ReadyPour™ Luria Broth Agar (sterile)
Luria Broth Medium for Recovery (sterile)
Petri Plates (small & large)
Plastic micro-tipped transfer pipettes
Wrapped 10ml pipette (sterile)
Toothpicks (sterile)
Inoculating loops (sterile)
Microcentrifuge tubes
Explore genetic engineering and the central dogma with this elegant experiment
Transform E.coli with pFluoroGreen™ - a plasmid containing genes for ampicillin resistance and the green fluorescent protein
Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
Expose transformed cells to IPTG to demonstrate differential gene expression
New transformation protocol has high transformation efficiency = great results!
A-Level Biology - Microbiology, Genetics, Transformation of E-Coli
Full supporting materials included.
EDVOTEK YouTube Channel is full of useful resources.
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