Most molecules have a net charge within a pH range of 2 to 10. When the
pH is altered, the net charge on molecules can change drastically. In this Ion
Exchange Chromatography Kit, a mixture of two chemicals is absorbed onto a
solid support ion-exchange column and separated during elution under conditions
that influence their net charge.
Group
Size: for 10 lab groups
Storage:
Room temperature
Time
Required: Complete in 60-90 minutes
The kit includes:
Full
instructions
Ion
exchanger
Chemical
mixture
Potassium
Acetate buffer
Chromatography
columns
Reasons to Love
Understand
and practice ion exchange chromatography
Explore
how charge affects a molecule’s behaviour
Prepare
a column then alter its pH to isolate different molecules
Specially
formulated dyes allow for easy visualization and for (optional)
quantification using a spectrophotometer
Learning Outcomes
A-Level
Chemistry: Chromatography Techniques - Ion Exchange Chromatography