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Ion Exchange Chromatography Kit

Product Code : SCL-CLE-12726

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this Ion Exchange Chromatography Kit, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Group Size: for 10 lab groups
Storage: Room temperature
Time Required: Complete in 60-90 minutes

The kit includes:

Full instructions
Ion exchanger
Chemical mixture
Potassium Acetate buffer
Chromatography columns

Reasons to Love

Understand and practice ion exchange chromatography
Explore how charge affects a molecule’s behaviour
Prepare a column then alter its pH to isolate different molecules
Specially formulated dyes allow for easy visualization and for (optional) quantification using a spectrophotometer

Learning Outcomes